Identifying differentially expressed genes by scRNA-seq
Single-cell RNA sequencing (scRNA-seq) comprises a state-of-the-art technology for analysing the gene expression profile of individual cells from a complex or even a uniform specimen. Differential gene expression analysis and […]
UMAP
Uniform Manifold Approximation and Projection (UMAP) is a dimensionality reduction technique to construct graphs showing the complexity of specimens and how the cell clusters diverse between each other according to […]
Next generation sequencing basics – RNA
RNA-sequencing (RNA-seq) is a technique that utilises next-generation sequencing to investigate gene expression in cells by quantifying the presence and amount of RNA reads within a sample. This allows scientist […]
Experimental design for single cell analysis
It is challenging to create generalizable designs for single-cell transcriptomic experiments because each one requires the user to make informed decisions in order to obtain interpretable results. These include the […]
Integrating scRNA-seq and scATAC-seq data
Single cell RNA sequencing (scRNA-seq) has exemplified recent advances in the anlaysis of transcriptomic heterogeneity, enabling the characterisation of gene expression in individual cells. The utility of scRNA-seq data can […]
tSNE
t-distributed stochastic neighbor embedding or more commonly referred to as tSNE is technique used to reduce the dimensions of a data set, but why is this desired? Effective data visualization […]
Perfusion
Perfusion is a type of cultivation method for cells. It is very similar in operation to a continuous stirred tank except that there is a device on the outflow that […]
Mitochondria
Mitochondria are the powerhouse of the cell. Distinctively, they contain their own DNA termed Mitochondrial DNA (mtDNA). Each mammalian cell contains thousands of copies of mtDNA. When all copies within […]
FN-Silk (Biosilk)
FN-Silk is a self-assembling spider silk functionalized with a Fibronectin to promote cell adhesion used for advanced 3D cell culture. It has been shown to be non-immunogenic and biodegradable. Cells […]
Single cell transcriptomics – a gerneric explanation of common steps
Single-cell RNA sequencing (scRNA-seq) has significantly advanced our knowledge of biological systems by studying the single cell transcriptome. Compared to bulk RNA-seq, scRNA-seq is able to study cellular heterogeneity. BDTM […]
